Desalting column how does it work

Either way, the salts within the solution will be replaced, either by water or a different buffer salt. With gel filtration, buffer salts and other small molecules within the substance will slide through the resin beads. Faster molecules within the solution will be separated from the smaller and slower molecules, thereby separating the larger molecules out.

In a buffer exchange, the original buffer salts will be retained within the resin while the rest of the solution can then be removed. Buffer exchanges are frequently used to exchange one type of salt for a salt type that is more suitable to the current applications. This can occur before affinity chromatography, ion exchange, or electrophoresis. When dealing with desalting and buffer exchange, the column size of the gel filtration will be extremely important.

Columns that are too large will lead to the dilution of the protein sample whereas columns that are too small could potentially lead to insufficient separation. Gravity-flow columns, chromatography cartridges, and centrifuge columns may all be used throughout this process.

These columns represent differences in how the sample is moved through the filter matrix. Gravity-flow columns simply rely upon gravity to bring the molecules to rest within the resin. Cartridges are closed systems with forced liquid and centrifuge systems spin in order to force the samples through. This allows for larger proteins to be retained on the column. For a protein complex or large protein of interest, these columns can be used to remove larger contaminants. In addition to desalting and buffer exchange for proteins, we offer a range of desalting columns optimized for various molecular biology techniques.

Check out our selection guides to determine the optimal columns for your protocols. Spin Desalting Columns. Gravity Desalting Columns. Automated Desalting. Chromatography Columns.

Desalting Columns for Small Molecule Removal Desalting columns use the principle of size exclusion chromatography, also called gel filtration chromatography. Desalting Column Uses Desalting columns are used with both proteins and nucleic acids. Customer recognition. Customer-specific caching.

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Write your own review. Quickly remove salts and small peptides from large proteins high MW. Zeba Spin Desalting Columns and Plates are polypropylene devices containing a proprietary high-performance size-exclusion resin for effective removal of salts and other small molecules Benefits: High recovery — Read more. Reviews Description. Great column for desalting protein from 8M guanidine!